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MLE-12 cells were exposed to 0 or 8% CSE for 48 h after cells were transfected with an miR-125a-5p inhibitor or a con inhibitor for 24 h

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These estimates suggest a possible threshold effect whereby at higher frequency levels of vaping, the likelihood of initiating and progressing to more frequent smoking is increased, but at low levels of vaping frequency the likelihood of progression in smoking frequency is not changed